September 3, 2020

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The techniques for generating transgenic fish have been established for numerous species, including some species suitable for developmental studies—such as the zebrafish and medaka—and many commercially important species. The high level of PhGH expression was found in the liver, pituitary, intestine and brain; smaller amounts were detectable in muscle, spleen, kidneys, heart, and stomach, caudal fin, gill and eyes, range from 0.02-0.75 PhGH/-actin mRNA. One of the factors that influence the success of catfish spawning, including male broodstock gonad fertility determine differences in the number of the sperm produced. Importantly, a long-term in vitro culture system for SSCs has been now established for some fish species. (For color version of this figure, the reader is referred to the web version of this book.). Fish pigmentation with "poster" colors is an aphrodisiac to wild fish and may even provide protection from predators in certain light conditions [3,4], or the pigment fluorescence may signal toxic defence as in the stinging sea anemone from which the glofish transgene was prepared and in that way discourage predators. Two transcranial injections produced broad expression throughout the brain. We also employed CRISPR-Cas9 to generate double mutant fish of sp7a;mstnba with high efficiencies in a single step. Bell J, Tocher D, Henderson J, Dick J and Crampton V. Altered fatty acid compositions in Atlantic salmon (. In contrast clear bands of hybridization were evident in all tissues, except for blood cells, of the AFP transgenics with heart, liver and brain tissue showing the highest levels of mRNA expression. Transgenic fish were found to have significantly higher numbers of small-diameter muscle fibres in both the dorsal and lateral region of the somitic muscle, suggesting that they grow by greater rates of hyperplasia relative to slower growing nontransgenic fish. However, before such fish are allowed to be marketed as food they must receive government regulatory approval. (C) Two-photon confocal image of the pancreas from a live 5 dpf larval fish, revealing the membrane localization of eGFP-KRASG12V protein. The mechanism of action of DNA vaccines, including the role of the innate immune responses induced shortly after DNA vaccination in the activation of the adaptive immunity providing longer term specific protection, is still not fully understood. 306th Semester (A) 2. The MThGH sequences were organized in head-to-tail tandem arrays that are characteristic of foreign genes integrated into recipient cell genomes. [45 Clone or several clones can be specifically identified for presence of desired alteration, expended in vitro and transplanted in vivo. Overall, 170 DEPs were identified in the 3 GM soybean lines with the same parents, but 232 DEPs were identified in the 3 natural soybean lines. Fertilized eggs were transferred to a Petri dish containing Holtfreter's solution Injection volume was calibrated and then guide RNAs/Cas9 targeting the toll/interleukin 1 receptor domain-containing adapter molecule (TICAM 1) gene and rhamnose binding lectin (RBL) gene were microinjected into the yolk of one-cell embryos. Scientists Julio E. Pérez and Mauro Nirchio of Venezuala along with Juan A. Gomez of Panama commented in Nature: "However, if the aquaculture industry is going to reduce the pressure on wild fish stocks and provide food for the world's growing population, substantial changes must be made by governments, the private sector and international funding agencies. For example, a gene involved in regulating erythropoiesis was identified by screening a cDNA library made from GATA-1/GFP expressing cells purified by fluorescence-activated cell sorting (Long et al., 2000a). The traditional approach for gene editing with zinc-finger nucleases (ZFNs) in fish has been microinjection of mRNA. The isolation and characterization of these cells in fish have been so far limited by the lack of specific molecular markers, hampering the high SSCs biotechnological potential for aquaculture. Optimization of protocols for microinjection-based delivery of cryoprotective agents into Japanese whiting Sillago japonica embryos, Impact of Lactobacillus casei BL23 on the Host Transcriptome, Growth and Disease Resistance in Larval Zebrafish, Proteomic comparison of selective breeding and growth hormone transgenesis in fish: Unique pathways to enhanced growth, Self-Reprogramming of Spermatogonial Stem Cells into Pluripotent Stem Cells without Microenvironment of Feeder Cells, Application of Zebrafish and Knockdown Technology to Define Progranulin Neuronal Function, Molecular characterization and expression patterns of Nanog gene validating its involvement in the embryonic development and maintenance of spermatogonial stem cells of farmed carp, Labeo rohita, Growth hormone transgenesis in coho salmon disrupts muscle immune function impacting cross-talk with growth systems, Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering, Targeted disruption of sp7 and myostatin with CRISPR-Cas9 results in severe bone defects and more muscular cells in common carp, Phytase in non-ruminant animal nutrition: A critical review on phytase activities in the gastrointestinal tract and influencing factors, Coho salmon (Oncorhynchus kisutch) transgenic for growth hormone gene construct exhibit increased rates of hyperplasia and detectable levels of differential gene expression, PhOTO Zebrafish: A Transgenic Resource for In Vivo Lineage Tracing during Development and Regeneration, Gene Editing in Channel Catfish via Double Electroporation of Zinc-Finger Nucleases: Methods and Protocols, Generation of albino medaka (Oryzias latipes) by CRISPR/Cas9, A Zebrafish Heart Failure Model for Assessing Therapeutic Agents, Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing, Transgenic expression of tilapia piscidin 3 (TP3) in zebrafish confers resistance to Streptococcus agalactiae, Progress and biotechnological prospects in fish transgenesis, Designer Probiotics: Paving the Way to Living Therapeutics, Production of fertile sperm from in vitro propagating enriched spermatogonial stem cells of farmed catfish, Clarias batrachus, Bioengineered probiotics as a new hope for health and diseases: An overview of potential and prospects, Differentiation of zebrafish spermatogonial stem cells to functional sperm in culture, Effect of the probiotic Lactobacillus rhamnosus on the expression of genes involved in European eel spermatogenesis, First evidence of molecular characterization of rohu carp Sox2 gene being expressed in proliferating spermatogonial cells, Biology and identity of fish spermatogonial stem cell, Techniques of chromosome manipulation in rainbow trout: a new evaluation with Karyology, Expression and fate of CAT reporter gene microinjected into fertilized medaka (Oryzias latipes) eggs in the form of plasmid DNA, recombinant phage particles and its DNA, Transfer of the Metallothionein-Human Growth Hormone Fusion Gene into Channel Catfish, 6 Transgenic Fish in Aquaculture and Developmental Biology, Novel gene transfer into the fertilized eggs of gold fish (Carassius auratus L. 1758), Generation of transgenic zebrafish with liver-specific expression of EGFP-Lc3: A new in vivo model for investigation of liver autophagy, Production of zebrafish offspring from cultured spermatogonial stem cells, Tilapia hepcidin (TH)2-3 as a transgene in transgenic fish enhances resistance to Vibrio vulnificus infection and causes variations in immune-related genes after infection by different bacterial species, Germline transgenesis of zebrafish using the medaka Tol1 transposon system, Antifreeze protein gene transfer in Atlantic salmon, Electroporation: a method for transferring genes into the gametes of zebrafish (Brachydanio rerio), channel catfish (Ictalurus punctatus), and common carp (Cyprinus carpio), Growth Enhancement in Transgenic Atlantic Salmon by the Use of an “All Fish” Chimeric Growth Hormone Gene Construct, A transgene containing lacZ is expressed in primary sensory neurons in zebrafish, Electroporation as a new technique for producing transgenic fish, Gene transfer, expression and inheritance of PRSV-rainbow trout-GH cDNA in the common carp,Cyprinus carpio (Linnaeus), Dramatic Growth of Mice That Develop from Eggs Microinjected with Metallothionein-Growth Hormone Fusion Genes, Expression of the antifreeze protein gene in transgenic goldfish (Carassius auratus) and its implication in cold adaption, Electroporation as an effective means of introducing DNA into abalone (Haliotis rufescens) embryos, Possible Ecological Risks of Transgenic Organism Release When Transgenes Affect Mating Success: Sexual Selection and the Trojan Gene Hypothesis, Erratum: Characterization and multi-generational stability of the growth hormone transgene (EO-1α) responsible for enhanced growth rates in Atlantic Salmon (Transgenic Research DOI: 10.1007/s11248-006-0020-5), Changes in hepatic gene expression related to innate immunity, growth and iron metabolism in GH-transgenic amago salmon (Oncorhynchus masou) by cDNA subtraction and microarray analysis, and serum lysozyme activity, Tissue specific expression of antifreeze protein and growth hormone transgenes driven by the ocean pout (Macrozoarces americanus) antifreeze protein OP5A gene promoter in Atlantic salmon (Salmo salar), In vivo synthesis of meganuclease for generating transgenic zebrafish Danio rerio. 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